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Chromatographic detection of squalene

 
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Fred
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PostPosted: Sun Dec 19, 2004 9:39 pm    Post subject: Chromatographic detection of squalene Reply with quote

Most liquid chromatographs detect chemical compounds by measuring their absorption of ultraviolet light. Compounds such as squalene have double-bonds which allow them to absorb ultraviolet light and, therefore, be detected. However, the double bonds in squalene are "non-conjugated", that is, non-alternating with single bonds. Logically, conjugated and non-conjugated double bonds will absorb ultraviolet light at different wavelengths and, by chosing the "correct" wavelength, the detection of squalene can be avoided. (see p. 177).
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PostPosted: Mon Dec 20, 2004 4:22 am    Post subject: Reply with quote

It's true that UV light is used to detect squalene in LC analysis. However, the maim paremeter is not the wavelength of UV light but the retention time in the column. Standards are run with known amounts of squalene first. Then, once a reliable detection limit has been reached, the real samples are run. Anything with an identical retention time is an identical molecule - and the concentration can be reliably determined by the amount of UV absorption.
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